Dr. Bindu Subhadra
Assistant Professor of Biology
Courses Taught
BIOL 2054: Microbiology for Health Sciences
BIOL 3054: Microbiology
Research Interest
Our lab explores the regulation of multidrug resistance, cell-to-cell communication (also known as quorum sensing) system, pathogenesis, and virulence in infectious disease-causing pathogens. We have a particular interest in the nosocomial pathogen, Acinetobacter. We employ a multidisciplinary approach that integrates molecular microbiology, cell biology and bioinformatics tools to gain a comprehensive understanding of Acinetobacter strains. Our goal is to decipher the mechanisms that enable these strains to thrive and develop strategies to tackle them effectively. In addition, we are interested in the identification of natural and synthetic compounds that can serve as potent antimicrobial agents for treating Acinetobacter infections. Our research endeavors are focused on advancing the field of infectious disease management and contributing to the development of novel therapeutic strategies. We welcome collaborations and discussions that align with our research interests.
Research Interest Keywords:
· Multidrug resistance
· Acinetobacter
· Quorum sensing
· Virulence
· Small RNA (sRNA)
· Infectious disease pathogens
· Antimicrobial agents
Education
Ph.D. Molecular Microbiology, Paichai University, Korea, 2012
M.Sc. Biotechnology, Cochin University of Science and Technology, India, 2003
B.Sc. Zoology, University of Kerala, India, 2001
Professional Experience
May 2023-July 2024 Research Scientist, Department of Veterinary Biomedical
Sciences, College of Veterinary Medicine, Long Island University,
Brookville, New York, USA.
Jan 2023-May 2023 Post-doctoral Research Scientist, Department of Veterinary Biomedical
Sciences, College of Veterinary Medicine, Long Island University,
Brookville, New York, USA.
Dec 2019-Dec 2022 Post-doctoral Research Associate, Department of Veterinary Biomedical
Sciences, College of Veterinary Medicine, Long Island University,
Brookville, New York, USA.
Jan 2015-Oct 2019 Post-doctoral Researcher, Department of Microbiology and Medical
Science, College of Medicine, Chungnam National University, Daejeon,
Korea.
Nov 2012-Nov 2014 Post-doctoral Researcher, Synthetic Biology and Bioengineering
Research Center, Korea Research Institute of Bioscience and
Biotechnology, Daejeon, Korea.
Jul 2004-Aug 2007 Research Assistant, Histopathology Lab, Sree Chitra Tirunal Institute
for Medical Sciences and Technology, Poojappura, Trivandrum, Kerala,
India.
Jul 2003-Mar 2004 Lecturer for Master’s and Bachelor’s students, Al-Azhar
College of Arts and Science, Thodupuzha, Kerala, India.
Publications
1. Rahul K, Makwana P, Ito K, Subhadra B, Kumar D, Pappachan A. Electronic Wastes: It’s Impact on Insects. In Electronic Wastes: Impact on Health, Animals, and the Environment. CRC Press, Taylor & Francis Group, Boca Raton, FL. (Accepted for publication)
2. Subhadra B, Sarshar M. Editorial on the Research Topic: Small non-coding RNAs in Gram negative bacteria. Front Cell Infect Microbiol 2024; 14:1426124.
3. Cao D, Subhadra B, Lee YJ, Thoresen M, Cornejo S, Woolums A and Inzana T. The contribution of Hfq to gene regulation and virulence in Histophilus somni. Infect Immun 2024; 92: e00038-24.
4. Subhadra B, Cao D, Jensen R, Caswell C, Inzana TJ. Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336. PloS ONE 2023; 18(5): e0286158.
5. Makwana P, Rahul K, Ito K, Subhadra B. Diversity of Antimicrobial Peptides in Silkworm. Life 2023; 13: 1161.
6. Gondil VS, Subhadra B. Biofilms and their role on diseases. BMC Microbiology 2023; 23:203.
7. Subhadra B. Special Issue: Biofilm composition and Applications. Coatings 2022; 12:1026.
8. Pan Y, Subhadra B, Sandal I, Dickerman A, Inzana TJ. The role of uspE in virulence and biofilm formation by Histophilus somni. Vet Microbiol 2021; 263:109267.
9. Subhadra B, Surendran S, Lim BR, Yim JS, Kim DH, Woo K, Kim HJ, Oh MH, Choi CH. The osmotic stress response operon betIBA is under the functional regulation of BetI and the quorum sensing regulator AnoR in Acinetobacter nosocomialis. J Microbiol 2020; 58(6): 519–29.
10. Subhadra B, Surendran S, Lim BR, Yim JS, Kim DH, Woo K, Kim HJ, Oh MH, Choi CH. Regulation of the AcrAB efflux system by the quorum-sensing regulator AnoR in Acinetobacter nosocomialis. J Microbiol 2020; 58(6): 507–18.
11. Surendran S, Subhadra B, Woo K, Park HS, Kim DH, Oh MH, Choi CH. Effects of exogenous N-Acyl-Homoserine lactones on biofilm formation and motility in Acinetobacter nosocomialis. J Bacteriol Virol 2020; 50(2): 97-106.
12. Lee C, Subhadra B, Choi HG, Suh HW, Uhm HS, Kim HJ. Inactivation of Mycobacteria by radicals from non-thermal plasma jet. J Microbiol Biotechnol 2019; 29(9):1401-11.
13. Subhadra B, Surendran S, Kim DH, Woo K, Oh MH, Choi CH. The transcription factor NemR is an electrophile-sensing regulator important for the detoxification of reactive electrophiles in
Acinetobacter nosocomialis. Res Microbiol 2019; 170(3):123-30.
14. Subhadra B, Surendran S, Lim BR, Yim JS, Kim DH, Woo K, Han K, Oh MH, Choi CH. Complete genome sequence and phylogenetic analysis of nosocomial pathogen Acinetobacter nosocomialis strain NCTC 8102. Genes Genom 2019; pp 1-13.
15. Subhadra B, Oh MH, Choi CH. RND efflux pump systems in Acinetobacter, with special emphasis on their role in quorum sensing. J Bacteriol Virol 2019; 49(1):1-11.
16. Kwon KK, Lee DH, Kim SJ, Choi SL, Rha E, Yeom SJ, Subhadra B, Lee J, Jeong KJ, Lee SG. Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds. Sci Rep 2018; 8:2659.
17. Kim SK, Kim SH, Subhadra B, Woo SG, Rha E, Kim SW, Kim H, Lee DH, Lee SG. A genetically encoded biosensor for monitoring isoprene production in engineered Escherichia coli. ACS Synth Biol 2018; 7(10):2379-90.
18. Subhadra B, Kim DH, Woo K, Surendran S, Choi CH. Control of biofilm formation in healthcare: Recent advances exploiting quorum-sensing interference strategies and multidrug efflux pump inhibitors. Materials 2018; 11(9):1676.
19. Subhadra B, Kim J, Kim DH, Woo K, Oh MH, Choi CH. Local repressor AcrR regulates AcrAB efflux pump required for biofilm formation and virulence in Acinetobacter nosocomialis. Front Cell Infect Microbiol 2018; 8:270.
20. Subhadra B, Kim DH, Kim J, Woo K, Sohn KM, Kim HJ, Han K, Oh MH, Choi CH. Complete genome sequence of uropathogenic Escherichia coli isolate UPEC 26-1. Genes Genom 2018; 40(6):643-55.
21. Kim DH, Subhadra B*, Kang HY, Woo K, Kim J, Woo K, Son YJ, Sohn KM, Kim HJ, Han K, Oh MH, Choi CH. Virulence properties of uropathogenic Escherichia coli isolated from children with urinary tract infection in Korea. Genes Genom 2018; 40(6):625-34 *Co-first author.
22. Kim SW, Subhadra B, Whang J, Back YW, Bae HS, Kim HJ, Choi CH. Clinical Mycobacterium abscessus strain inhibits autophagy flux and promotes its growth in murine macrophages. Pathog Dis 2017; 75(8) ftx107.
23. Subhadra B, Choi CH. Autophagy in Mycobacterium abscessus infection. J Bacteriol Virol 2017; 47(3):122.
24. Subhadra B, Oh MH, Choi CH. Simple method for markerless gene deletion in multidrug resistant Acinetobacter baumannii. J Bacteriol Parasitol 2017; 8:4.
25. Subhadra B, Oh MH, Choi CH. Quorum sensing in Acinetobacter: with special emphasis on
antibiotic resistance, biofilm formation and quorum quenching. AIMS Microbiol 2016; 2(1):27-41.
26. Lee JH, Subhadra B*, Son YJ, Kim DH, Park HS, Kim JM, Koo SH, Oh MH, Kim HJ, Choi CH. Phylogenetic group distributions, virulence factors and antimicrobial resistance properties of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in South Korea. Lett Appl Microbiol 2016; 62(1):84-90 *Co-first author.
27. Subhadra B, Ray D, Han JY, Bae KH, Lee JK. Identification of the regulators binding to the upstream region of glxR of Corynebacterium glutamicum. J Microbiol Biotechnol 2015; 25(8):1216-26.
28. Lee SG, Lee DH, Kim SK, Kim SH, Gam J, Rha E, Kim H, Choi SL, Choi E, Subhadra B. Method for screening and quantifying isoprene biosynthesis enzyme activity (PCT - Appl. No. PCT/KR2014/001360). Pub. No.: WO/2014/129808; Publication date - 28.08.2014.
29. Subhadra B and Lee JK. Elucidation of the regulation of ethanol catabolic genes and ptsG using a glxR and adenylate cyclase gene (cyaB) deletion mutants of Corynebacterium glutamicum ATCC 13032. J Microbiol Biotechnol 2013; 23(12):1683-90.
30. Park SY, Moon MW, Subhadra B and Lee JK: Functional characterization of the glxR deletion mutant of Corynebacterium glutamicum ATCC 13032: involvement of GlxR in acetate metabolism and carbon catabolite repression. FEMS Microbiol Lett 2010; 304(2):107-15.
31. Cha PH, Park SY, Moon MW, Subhadra B, Oh TK, Kim E, Kim JF and Lee JK. Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032. Appl Microbiol Biotechnol 2010; 85(4):1061-8.
32. Bindu S, Sanosh KP, Smetana K, Balakrishnan A and Kim TN. An in vivo evaluation of ultra-fine grained titanium implants. J Mater Sci Technol 2009; 25(4):556-60.
Professional webpages
Google scholar: https://scholar.google.com/citations?view_op=list_works&hl=en&user=EeDSg2cAAAAJ
ResearchGate: https://www.researchgate.net/profile/Bindu_Subhadra3